This site contains information about prescription peptide therapy protocols intended for adults. You must be 18 years of age or older to enter.
By entering, you confirm you are at least 18 years old and agree to our Terms of Service. All protocols require a valid physician prescription.
The upstream gatekeeper of the entire reproductive axis — activates GnRH neurons in the hypothalamus to stimulate pulsatile LH/FSH release and downstream testosterone production. Studied for hypogonadism, fertility support, and HPG axis restoration without suppressing endogenous hormone production.
Kisspeptin (encoded by the KISS1 gene, also called metastin) is a hypothalamic neuropeptide that acts on GPR54 receptors — the primary gate through which the reproductive hormone cascade begins. Without kisspeptin signaling, GnRH neurons do not fire. With it, they fire in pulses, driving the downstream chain of LH and FSH release from the pituitary, and ultimately testosterone and estrogen production from the gonads.
This upstream position makes kisspeptin fundamentally different from exogenous testosterone or even hCG/LH injections. Rather than bypassing the HPG (hypothalamic-pituitary-gonadal) axis, kisspeptin activates it from the top — preserving the entire feedback loop and the natural pulsatile testosterone rhythm that exogenous testosterone destroys. It is this property that makes it a subject of intense research interest in male hypogonadism and fertility contexts.
Kisspeptin-10 is the 10-amino acid C-terminal fragment of kisspeptin-54, the full-length protein. It retains full receptor binding activity and is the most commonly used form in research. Human infusion studies have reliably shown LH pulsatility increases within minutes of administration.
Kisspeptin sits at the top of the reproductive hormone hierarchy. Every downstream effect traces back to this single receptor activation:
In men with hypogonadotropic hypogonadism (low testosterone from inadequate GnRH/LH signaling), kisspeptin can restore the upstream signal. Multiple human studies show acute and sustained LH pulsatility responses.
Unlike exogenous testosterone, which suppresses LH/FSH and shuts down sperm production, kisspeptin stimulates both testosterone and the gonadotropins needed for spermatogenesis — a meaningful distinction for men concerned with fertility.
Post-TRT recovery or post-cycle restoration of natural testosterone production is an area of active research interest. Kisspeptin's upstream position makes it a candidate for restarting suppressed HPG axis function.
In women, kisspeptin plays a central role in ovulation timing and the LH surge. Research covers ovulation induction, polycystic ovary syndrome, and hypothalamic amenorrhea.
IV and SC kisspeptin studies in humans reliably increase LH pulse frequency and amplitude within minutes. This acute, measurable response makes it one of the better-characterized hypothalamic research peptides.
Kisspeptin has no direct androgenic activity — it does not bind androgen receptors and cannot cause exogenous testosterone-type suppression. Its effects are entirely mediated through the natural endocrine cascade.
Kisspeptin dosing in human research has primarily used IV infusion (for acute LH pulse studies) or SC injection. The subcutaneous SC route is the practical research model. Pulsatile administration — mimicking the natural GnRH pulse pattern — appears superior to continuous dosing based on available data.
| Protocol | Dose | Frequency | Notes |
|---|---|---|---|
| Standard SC | 1–10mcg/kg SC | 1–2x daily | Lower end (1–2mcg/kg) for maintenance HPG axis support. Higher end (5–10mcg/kg) for acute stimulation protocols. Weight-based dosing reflects human research methodology. |
| Fixed dose (common) | 10mcg SC | Once daily to twice daily | Many researcher protocols simplify to a 10mcg fixed SC dose. Morning administration is most common; some use twice-daily to approximate pulsatile pattern. |
| Cycle length | — | 4–12 weeks | Longer cycles (8–12 weeks) are used in fertility restoration contexts. Cycling allows assessment of endogenous axis response before continuing. |
IV and SC kisspeptin reliably increases LH pulse amplitude and frequency within minutes in humans. The response is dose-dependent and reproducible — one of the most consistently demonstrated acute neuroendocrine effects in hypothalamic research peptides.
KISS1R activates phospholipase C (PLC) via Gq/11α coupling, triggering intracellular calcium release and GnRH neuron depolarization. GPR54 knockout animals are infertile and fail puberty — establishing this receptor as essential, not supplementary, to reproductive function.
Peripheral kisspeptin administration has been demonstrated to cross the blood-brain barrier — meaning SC injection reaches hypothalamic KISS1R directly. This validates the peripheral SC route as equivalent to central delivery for clinical purposes.
Kisspeptin administration to healthy men enhances insulin secretion in response to an IV glucose load — without influencing fasting insulin levels. This selectivity suggests kisspeptin participates in meal-responsive insulin regulation through a "trihormonal regulatory circuit" linking the hypothalamus, pancreas, and liver. (PMC11006622, 2024)
Kisspeptin is typically used as a standalone HPG axis research peptide. It is not appropriate to combine with exogenous testosterone (the point of kisspeptin is to preserve the axis that exogenous testosterone suppresses). Complementary non-hormonal stacks:
Do not combine with exogenous androgens, SARMs, or other HPG-suppressing compounds — kisspeptin's benefit is preserving the axis these compounds suppress.
Third-party COA, Finnrick-verified purity, lyophilized for long-term stability. Ships within 24 hours.
Start Protocol Intake ← All ProtocolsMedical Disclaimer: The information on this page is for educational purposes only and does not constitute medical advice. All protocols require evaluation and prescription by a licensed physician. You should consult a qualified healthcare provider before starting any new medical protocol. Individual results vary. Cinch Bio is not a pharmacy and does not dispense medications — all prescriptions are issued by independent licensed physicians and filled by licensed 503A compounding pharmacies.